Filipa A. Vicente (Author), Igor Plazl (Author), Sónia P. M. Ventura (Author), Polona Žnidaršič Plazl (Author)

Abstract

Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidics-based separation and purification of proteins and nucleic acids with the focus on liquid–liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes.

Keywords

mikropretočni sistemi;biomakromolekule;dvofazni vodni sistemi;zaključni procesi;microflow systems;biomacromolecules;aqueous two-phase systems;downstream processing;

Data

Language: English
Year of publishing:
Typology: 1.02 - Review Article
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
UDC: 66.02
COBISS: 1538561987 Link will open in a new window
ISSN: 1463-9262
Views: 293
Downloads: 110
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: mikropretočni sistemi;biomakromolekule;dvofazni vodni sistemi;zaključni procesi;
Type (COBISS): Article
Pages: str. 4391-4410
Volume: ǂVol. ǂ22
Issue: ǂiss. ǂ14
Chronology: 21 Jul. 2020
DOI: 10.1039/c9gc04362d
ID: 12907181