Bojan Papić (Author), Majda Golob (Author), Irena Zdovc (Author), Darja Kušar (Author), Jana Avberšek (Author)

Abstract

Staphylococcus pseudintermedius is a common cause of skin and soft tissue infections in dogs but can also cause infections in cats and humans. The frequency of methicillin-resistant S. pseudintermedius (MRSP) strains is increasing worldwide. Here, we obtained 43 MRSP isolates from dogs (n = 41), one cat (n = 1) and the small animal clinic environment (n = 1) in Slovenia from the period 2008–2018, which underwent whole-genome sequencing (WGS) and antimicrobial susceptibility testing. Five sequence types (STs) were identified, with ST71 (32/43) and ST551 (8/43) being the predominant. In Slovenia, ST551 was first detected in 2016, whereas a decrease in the frequency of ST71 was observed after 2015. All isolates were multidrug-resistant and most antimicrobial-resistant phenotypes could be linked to acquisition of the corresponding resistance genes or gene mutations. Core-genome multilocus sequence typing (cgMLST) revealed several potential MRSP transmission routes: (i) between two veterinary clinics by a single MRSP-positive dog, (ii) between the environment of a veterinary clinic and a dog, and (iii) between a canine and a feline patient through the contaminated environment of a veterinary clinic. Of the six dogs that were additionally sampled from 14 days to five months after the initial sampling, each harbored the same MRSP strain, suggesting a limited within-host diversity of MRSP in symptomatic dogs. The present results highlight the importance of MRSP-positive dogs in the spread of veterinary care-associated MRSP infections and call for the implementation of strict control measures to reduce MRSP contamination in veterinary clinic environments originating from animal-contact surfaces.

Keywords

antimicrobial susceptibility testing;epidemiology;methicillin-resistant Staphylococcus pseudintermedius (MRSP);small animal clinic;whole-genome sequencing (WGS);

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL VF - Veterinary Faculty
UDC: 636.09:579
COBISS: 64516867 Link will open in a new window
ISSN: 1873-2542
Views: 379
Downloads: 109
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Other data

Secondary keywords: Epidemiology;Whole Genome Sequencing;Veterinary Medicine;
Type (COBISS): Article
Pages: art. 109119, str. 1-7
Issue: ǂVol. ǂ258
Chronology: 2021
DOI: 10.1016/j.vetmic.2021.109119
ID: 12994073