Bojan Papić (Avtor), Majda Golob (Avtor), Irena Zdovc (Avtor), Darja Kušar (Avtor), Jana Avberšek (Avtor)

Povzetek

Staphylococcus pseudintermedius is a common cause of skin and soft tissue infections in dogs but can also cause infections in cats and humans. The frequency of methicillin-resistant S. pseudintermedius (MRSP) strains is increasing worldwide. Here, we obtained 43 MRSP isolates from dogs (n = 41), one cat (n = 1) and the small animal clinic environment (n = 1) in Slovenia from the period 2008–2018, which underwent whole-genome sequencing (WGS) and antimicrobial susceptibility testing. Five sequence types (STs) were identified, with ST71 (32/43) and ST551 (8/43) being the predominant. In Slovenia, ST551 was first detected in 2016, whereas a decrease in the frequency of ST71 was observed after 2015. All isolates were multidrug-resistant and most antimicrobial-resistant phenotypes could be linked to acquisition of the corresponding resistance genes or gene mutations. Core-genome multilocus sequence typing (cgMLST) revealed several potential MRSP transmission routes: (i) between two veterinary clinics by a single MRSP-positive dog, (ii) between the environment of a veterinary clinic and a dog, and (iii) between a canine and a feline patient through the contaminated environment of a veterinary clinic. Of the six dogs that were additionally sampled from 14 days to five months after the initial sampling, each harbored the same MRSP strain, suggesting a limited within-host diversity of MRSP in symptomatic dogs. The present results highlight the importance of MRSP-positive dogs in the spread of veterinary care-associated MRSP infections and call for the implementation of strict control measures to reduce MRSP contamination in veterinary clinic environments originating from animal-contact surfaces.

Ključne besede

antimicrobial susceptibility testing;epidemiology;methicillin-resistant Staphylococcus pseudintermedius (MRSP);small animal clinic;whole-genome sequencing (WGS);

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL VF - Veterinarska fakulteta
UDK: 636.09:579
COBISS: 64516867 Povezava se bo odprla v novem oknu
ISSN: 1873-2542
Št. ogledov: 379
Št. prenosov: 109
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarne ključne besede: Epidemiology;Whole Genome Sequencing;Veterinary Medicine;
Vrsta dela (COBISS): Članek v reviji
Strani: art. 109119, str. 1-7
Zvezek: ǂVol. ǂ258
Čas izdaje: 2021
DOI: 10.1016/j.vetmic.2021.109119
ID: 12994073