investigation of chemical composition and antioxidant activity
Abstract
With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R 2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.
Keywords
green solvents;chamomile;yarrow;St. John's wort;curry plant;square-wave voltammetry;chromatography;
Data
Language: |
English |
Year of publishing: |
2021 |
Typology: |
1.01 - Original Scientific Article |
Organization: |
UL ZF - University College of Health Studies |
UDC: |
543.544 |
COBISS: |
77584387
|
ISSN: |
1420-3049 |
Views: |
225 |
Downloads: |
54 |
Average score: |
0 (0 votes) |
Metadata: |
|
Other data
Secondary keywords: |
zelena topila;kamilica;rman;šentjanževka;curry;voltametrija s kvadratnimi valovi;kromatografija; |
Type (COBISS): |
Article |
Pages: |
str. 1-18 |
Volume: |
ǂVol. ǂ26 |
Issue: |
ǂiss. ǂ18 |
Chronology: |
2021 |
DOI: |
10.3390/ molecules26185697 |
ID: |
13534224 |