investigation of chemical composition and antioxidant activity
Lara Čižmek (Avtor), Mojca Bavcon Kralj (Avtor), Rozelindra Čož-Rakovac (Avtor), Dmitry M. Mazur (Avtor), Nikolai Ul'yanovskii (Avtor), Marko Likon (Avtor), Polonca Trebše (Avtor)

Povzetek

With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatographyhigh-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R 2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.

Ključne besede

green solvents;chamomile;yarrow;St. John's wort;curry plant;square-wave voltammetry;chromatography;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL ZF - Zdravstvena fakulteta
UDK: 543.544
COBISS: 77584387 Povezava se bo odprla v novem oknu
ISSN: 1420-3049
Št. ogledov: 225
Št. prenosov: 54
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarne ključne besede: zelena topila;kamilica;rman;šentjanževka;curry;voltametrija s kvadratnimi valovi;kromatografija;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-18
Letnik: ǂVol. ǂ26
Zvezek: ǂiss. ǂ18
Čas izdaje: 2021
DOI: 10.3390/ molecules26185697
ID: 13534224