diplomsko delo
Taja Vita Petrović (Author), Neža Čadež (Reviewer), Sonja Smole Možina (Mentor)

Abstract

Spomladanska resa (Erica carnea) je zimzelena rastlina, ki ji pripisujejo številne biološke aktivnosti, med njimi tudi protibakterijsko delovanje. V diplomski nalogi smo preverili protibakterijsko in protiadhezijsko delovanje etanolnih izvlečkov spomladanske rese in kemijsko čistih učinkovin na izbrane testne mikroorganizme. Uporabili smo grampozitivni bakteriji Staphylococcus aureus in Listeria innocua ter gramnegativni bakteriji Escherichia coli in Pseudomonas fragi. Določili smo minimalno inhibitorno koncentracijo (MIK) in minimalno baktericidno koncentracijo (MBK) etanolnih izvlečkov in nekaterih kemijsko čistih učinkovin, ki jih vsebujejo etanolni izvlečki spomladanske rese. Rezultati so pokazali najboljši protibakterijski učinek etanolnih izvlečkov proti bakteriji S. aureus, MIK je znašala 1 mg/ml. Najboljši protibakterijski učinek kemijsko čiste učinkovine je imel kvercetin, z najnižjimi vrednostmi proti grampozitivnim bakterijam (MIK 0,25 mg/ml; MBK 1 mg/ml). Ugotovili smo tudi relativno nizke vrednosti MIK etanolnih izvlečkov proti bakteriji P. fragi (1-2 mg/ml). Te vrednosti so primerljive, ali v primeru kemijsko čistih učinkovin celo nižje od tistih za grampozitivne bakterije, na kar predvidoma vpliva specifična encimska aktivnost bakterij P. fragi. Določili smo tudi dobro protiadhezijsko aktivnost etanolnega izvlečka spomladanske rese, ki je adhezijo bakterije P. fragi na polistiren statistično značilno zmanjšal, povprečno za 52,5 %. Na podlagi rezultatov smo potrdili protibakterijsko delovanje etanolnih izvlečkov spomladanske rese proti izbranim testnim bakterijam, pri čemer so dobljene vrednosti MIK in protiadhezijske aktivnosti na bakterijo P. fragi zanimiva tema nadaljnjih študij.

Keywords

spomladanska resa;Erica carnea;protibakterijski učinek;minimalna inhibitorna koncentracija;minimalna baktericidna koncentracija;protiadhezijska aktivnost;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [T. V. Petrović]
UDC: 579.24/.26:547.9
COBISS: 97683203 Link will open in a new window
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Downloads: 35
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Other data

Secondary language: English
Secondary title: Antibacterial activity of ethanolic extracts of spring heath (Erica carnea)
Secondary abstract: Spring heath (Erica carnea) is an evergreen plant, with many biological activities, including antibacterial activity. In this thesis we checked the antibacterial and antiadhesion effect of ethanolic extracts of spring heath and some chemically pure active ingredients of these ethanolic extracts on selected target microorganisms. We used two gram positive target bacteria, Staphylococcus aureus and Listeria innocua, and two gram negative bacteria, Escherichia coli and Pseudomonas fragi. We determined the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of ethanolic extracts and some of their chemically pure active ingredients. The results showed the best antibacterial effect of ethanolic extracts against S. aureus (MIC 1 mg/ml) and the best antibacterial effect of quercetin, the chemically pure active substance, with the lowest values for gram positive bacteria (MIC 0,25 mg/ml; MBC 1 mg/ml). The results for gram negative P. fragi are interesting, due to relatively low MIC values (1-2 mg/ml), which are comparable or, in case of chemically pure active compounds, even lower than the values obtained for gram positive bacteria. Presumably, this is influenced by specific enzymatic activity of P. fragi. We also determined a statistically significant reduction (52,5 %) in the adhesion of P. fragi to polystyrene, after the addition of ethanol extract. We have confirmed the antibacterial effect of spring heath extract on selected target microorganisms and found that the obtained MIC values and antiadhesion activity for P. fragi are an interesting topic for additional studies.
Secondary keywords: spring heath;Erica carnea;antibacterial activity;minimal inhibitory concentration;minimal bactericidal concentration;antiadhesion activity;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Pages: VIII, 26 f., [1] f. pril.
ID: 14519300