magistrsko delo
Ivana Podlipnik (Author), San Hadži (Mentor), Franc Požgan (Thesis defence commission member), Jurij Lah (Thesis defence commission member)

Abstract

Za proteine obstajajo tako imenovane adicijske sheme, s pomočjo katerih je možno napovedati parcialno molsko toplotno kapaciteto denaturiranega (razvitega) stanja za poljuben protein na osnovi števila posameznih aminokislin v tem proteinu, kar je ključnega pomena za raziskave stabilnosti. V tem magistrskem delu sem se lotila raziskave aditivnosti parcialne toplotne kapacitete nukleotidov v DNK in razvoja enačbe, ki bi služila kot adicijska shema za oceno parcialne molske toplotne kapacitete denaturiranega (razvitega) stanja poljubnih enoverižnih DNK na podlagi merjenja parcialne molske toplotne kapacitete krajšim modelnim spojinam (DNK oligonukleotidov) z diferenčno dinamično kalorimetrijo. Določila sem prispevke za posamezne oligonukleotide (A, T, C, G) in tako parametrizirala enačbo za napoved parcialne molske toplotne kapacitete. Napovedane vrednosti se v grobem ujemajo s podatki, zato bi bilo potrebno parametre v enačbi izboljšati. Potencial za izboljšavo vidim v upoštevanju okolice oziroma najbližjih sosedov, ki imajo nezanemarljiv vpliv na prispevek posameznega nukleotida k parcialni molski toplotni kapaciteti.

Keywords

toplotna kapaciteta;adicijske sheme;diferenčna dinamična kalorimetrija;DSC;magistrska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [I. Podlipnik]
UDC: 577.31(043.2)
COBISS: 198709251 Link will open in a new window
Views: 74
Downloads: 12
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Other data

Secondary language: English
Secondary title: Determination of parcial molar heat capacity of DNA oligonucleotides
Secondary abstract: For proteins, so-called additive schemes exist, which allow predicting the partial molar heat capacity of the denatured (unfolded) state for any protein based on the number of individual amino acids in that protein, which is crucial for stability research. In this master's thesis, I undertook the investigation of the additivity of the partial heat capacity of nucleotides in DNA and the development of an equation that would serve as an additive scheme for estimating the partial molar heat capacity of denatured (unfolded) states of arbitrary single-stranded DNA based on measuring the partial molar heat capacity of shorter model compounds (DNA oligonucleotides) using differential scanning calorimetry (DSC). I determined the contributions for individual nucleotides (A, T, C, G) and as such parameterized the equation for predicting the partial molar heat capacity. The predicted values roughly match the data, indicating that the parameters in the equation need improvement. Potential for improvement lies in considering the surroundings or nearest neighbors, which have a significant influence on the contribution of individual nucleotides to the partial molar heat capacity.
Secondary keywords: DSC;DNA;heat capacity;addition scheme;Termodinamika;Univerzitetna in visokošolska dela;
Type (COBISS): Master's thesis/paper
Study programme: 1000375
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, smer Kemija
Pages: 1 spletni vir (1 datoteka PDF (40 str.))
ID: 24275728