ǂan ǂexploratory study
Tilen Seničar (Author), Andraž Kukovičič (Author), Valerija Zidarič (Author), Aleksander Mahnič (Author), Jernej Dolinšek (Author), Maja Rupnik (Author)

Abstract

Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13-18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva and fecal samples were compared with molecular profiling, and microorganisms displaying lysis zones on gluten-containing media (i.e., gluten-degrading microorganisms; GDMs) were isolated. In total, 45 gluten-degrading strains were isolated, belonging to 13 genera and 15 species, including Candida albicans and Veillonella. GDMs were more common in HVs compared to CD patients and more diverse in saliva compared to feces. In saliva, GDMs showed partial overlap between HVs and CD patients. Bacterial communities in fecal samples determined with amplicon sequencing significantly differed between CD patients and HVs. Overall, 7-46 of all operational taxonomic units (OTUs) per sample were below the detection limit in the fecal samples but were present in the cultivated samples, and mainly included representatives from Lactobacillus and Enterococcus. Furthermore, differences in fecal short-chain fatty-acid concentrations between CD patients and HVs, as well as their correlations with bacterial taxa, were demonstrated.

Keywords

gluten-degrading microorganisms;veillonella;candida;short-chain fatty acids;celiac disease;microbiota;sequencing;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM MF - Faculty of Medicine
Publisher: MDPI
UDC: 577
COBISS: 86065923 Link will open in a new window
ISSN: 2076-2607
Views: 0
Downloads: 8
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Other data

Secondary language: Slovenian
Secondary keywords: mikroorganizmi;kandida;kratkoverižne maščobne kisline;celiakija;mikrobiota;sekvenciranje;
Type (COBISS): Scientific work
Pages: str. 1-9
Volume: ǂVol. ǂ9
Issue: ǂiss. ǂ11
Chronology: Nov. 2021
DOI: 10.3390/microorganisms9112375
ID: 25290849