Comparison of microbial populations in saliva and feces from healthy and celiac adolescents with conventional and molecular approaches after cultivation on gluten-containing media

ǂan ǂexploratory study

Tilen Seničar (Avtor), Andraž Kukovičič (Avtor), Valerija Zidarič (Avtor), Aleksander Mahnič (Avtor), Jernej Dolinšek (Avtor), Maja Rupnik (Avtor)

Povzetek

Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13-18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva and fecal samples were compared with molecular profiling, and microorganisms displaying lysis zones on gluten-containing media (i.e., gluten-degrading microorganisms; GDMs) were isolated. In total, 45 gluten-degrading strains were isolated, belonging to 13 genera and 15 species, including Candida albicans and Veillonella. GDMs were more common in HVs compared to CD patients and more diverse in saliva compared to feces. In saliva, GDMs showed partial overlap between HVs and CD patients. Bacterial communities in fecal samples determined with amplicon sequencing significantly differed between CD patients and HVs. Overall, 7-46 of all operational taxonomic units (OTUs) per sample were below the detection limit in the fecal samples but were present in the cultivated samples, and mainly included representatives from Lactobacillus and Enterococcus. Furthermore, differences in fecal short-chain fatty-acid concentrations between CD patients and HVs, as well as their correlations with bacterial taxa, were demonstrated.

Ključne besede

gluten-degrading microorganisms;veillonella;candida;short-chain fatty acids;celiac disease;microbiota;sequencing;

Podatki

Jezik:	Angleški jezik
Leto izida:	2021
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM MF - Medicinska fakulteta
Založnik:	MDPI
UDK:	577
COBISS:	86065923
ISSN:	2076-2607
Št. ogledov:	0
Št. prenosov:	8
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	mikroorganizmi;kandida;kratkoverižne maščobne kisline;celiakija;mikrobiota;sekvenciranje;
Vrsta dela (COBISS):	Znanstveno delo
Strani:	str. 1-9
Letnik:	ǂVol. ǂ9
Zvezek:	ǂiss. ǂ11
Čas izdaje:	Nov. 2021
DOI:	10.3390/microorganisms9112375
ID:	25290849