Alja Ribič (Author), Janja Trček (Author)

Abstract

Acetic acid bacteria (AAB) are involved in food and beverage production bioprocesses, like those in vinegar and kombucha. They oxidize sugars and alcohols into various metabolites, resulting in the final products’ pleasant taste and aroma. The 16S rDNA amplicon metagenomics using Illumina technology is usually used to follow the microbiological development of these processes. However, the 16S rRNA gene sequences among different species of AAB are very similar, thus not enabling a reliable identification down to the species level but only to the genus. In this study, we have constructed primers for amplifying half of the 16S-23S rRNA gene internal transcribed spacer (ITS) for library construction and further sequencing using Illumina technology. This approach was successfully used to estimate the relative abundance of AAB species in defined consortia. Further application of this method for the analysis of different vinegar and kombucha samples proves it suitable for assessing the relative abundance of AAB species when these bacteria represent a predominant part of a microbial community.

Keywords

mikrobiota;konzorciji;ne zaključna dela;ITS metagenomics;microbiota;consortia;Acetobacter;Komagataeibacter;Novacetimonas;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FNM - Faculty of Natural Sciences and Mathematics
UDC: 579
COBISS: 196373763 Link will open in a new window
ISSN: 2076-2607
Views: 0
Downloads: 0
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Other data

Secondary language: Slovenian
Secondary keywords: Mikrobiologija;Bakterije;
Type (COBISS): Scientific work
Pages: 8 str.
Volume: ǂVol. ǂ12
Issue: ǂiss. ǂ5, [article no.] 1023
Chronology: 2024
DOI: 10.3390/microorganisms12051023
ID: 25839021