Alja Ribič (Avtor), Janja Trček (Avtor)

Povzetek

Acetic acid bacteria (AAB) are involved in food and beverage production bioprocesses, like those in vinegar and kombucha. They oxidize sugars and alcohols into various metabolites, resulting in the final products’ pleasant taste and aroma. The 16S rDNA amplicon metagenomics using Illumina technology is usually used to follow the microbiological development of these processes. However, the 16S rRNA gene sequences among different species of AAB are very similar, thus not enabling a reliable identification down to the species level but only to the genus. In this study, we have constructed primers for amplifying half of the 16S-23S rRNA gene internal transcribed spacer (ITS) for library construction and further sequencing using Illumina technology. This approach was successfully used to estimate the relative abundance of AAB species in defined consortia. Further application of this method for the analysis of different vinegar and kombucha samples proves it suitable for assessing the relative abundance of AAB species when these bacteria represent a predominant part of a microbial community.

Ključne besede

mikrobiota;konzorciji;ne zaključna dela;ITS metagenomics;microbiota;consortia;Acetobacter;Komagataeibacter;Novacetimonas;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FNM - Fakulteta za naravoslovje in matematiko
UDK: 579
COBISS: 196373763 Povezava se bo odprla v novem oknu
ISSN: 2076-2607
Št. ogledov: 0
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Mikrobiologija;Bakterije;
Vrsta dela (COBISS): Znanstveno delo
Strani: 8 str.
Letnik: ǂVol. ǂ12
Zvezek: ǂiss. ǂ5, [article no.] 1023
Čas izdaje: 2024
DOI: 10.3390/microorganisms12051023
ID: 25839021