Peter Krajnc (Author), Nermina Leber (Author), Dejan Štefanec (Author), Sandra Kontrec (Author), Aleš Podgornik (Author)

Abstract

Poly(glycidyl methacrylate-co-ethyleneglycol dimethacrylate) monolithic supports were prepared by radical polymerisation of the continuous phase of water in oil high internal phase emulsions. Morphology of monolithic materialswas studied by scanning electron microscopy and mercury intrusion porosimetry. The ratio of phase volume and the degree of crosslinking influenced the void size and pore size distribution of resulting polymers. Void sizes between 1 and 10 m were observed and average pore sizes around 100nm. Polymers with 60, 75, 80 and 90% pore volume were prepared and even samples with highest pore volume showed good mechanical stability. They were modified to bear weak-anion exchange groups and tested on the separation of standard protein mixture containing myoglobin, conalbumine and trypsin inhibitor. Good separation was obtained in a very short time similar to the separation obtained by commercial methacrylate monoliths. However, higher dispersion was observed. Bovine serum albumin dynamic binding capacity for monolith with 90% porosity was close to 9 mg/ml.

Keywords

organic chemistry;methacrylate monoliths;preparation;emulsion polymerisation;high porosity;high mechanical stability;polymer chromatographic supports;monolithic methacrylate supports;protein separation;polyHIPE;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC: 547:678.7
COBISS: 9370134 Link will open in a new window
ISSN: 0021-9673
Views: 1853
Downloads: 45
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Other data

Secondary language: English
Secondary keywords: organska kemija;metakrilatni monoliti;priprava;emulzijska polimerizacija;visoka poroznost;visoka mehanska stabilnost;polimerni kromatografski nosilci;monolitni metakrilatni nosilci;separacija proteinov;
URN: URN:SI:UM:
Pages: str. 69-73
Volume: ǂVol. ǂ1065
Issue: ǂiss. ǂ1
Chronology: 2005
ID: 8718472