Marko Marhl (Author), Marko Gosak (Author), Matjaž Perc (Author), C. Jane Dixon (Author), Anne K. Green (Author)

Abstract

In many non-excitable eukaryotic cells, including hepatocytes, ▫$Ca^{2+}$▫ oscillations play a key role in intra- and intercellular signalling, thus regulating many cellular processes from fertilisation to death. Therefore, understanding the mechanisms underlying these oscillations, and consequently understanding how they may be regulated, is of great interest. In this paper, we study the influence of reduced ▫$Ca^{2+}$▫ plasma membrane efflux on ▫$Ca^{2+}$▫ oscillations in hepatocytes. Our previous experiments with carboxyeosin show that a reduced plasma membrane ▫$Ca^{2+}$▫ efflux increases the frequency of ▫$Ca^{2+}$▫ oscillations, but does not affect the duration of individual transients. This phenomenon can be best explained by taking into account not only the temporal,but also the spatial dynamics underlying the generation of ▫$Ca^{2+}$▫ oscillations in the cell. Here we divide the cell into a grid of elements and treat the ▫$Ca^{2+}$▫ dynamics as a spatio-temporal phenomenon. By converting an existing temporal model into a spatio-temporal one, we obtain theoretical predictions that are in much better agreement with the experimental observations.

Keywords

celično signaliziranje;kalcijeve oscilacije;celične oscilacije;prostorsko-časovna dinamika;hepatociti;stohastične simulacije;cellular signalling;calcium oscillations;intracellular oscilations;spatio-temporal dynamics;hepatocytes;stochastic simulations;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FNM - Faculty of Natural Sciences and Mathematics
UDC: 519.83:53
COBISS: 16014344 Link will open in a new window
ISSN: 0022-5193
Views: 1159
Downloads: 34
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Other data

Secondary language: English
Secondary keywords: celično signaliziranje;kalcijeve oscilacije;celične oscilacije;prostorsko-časovna dinamika;hepatociti;stohastične simulacije;
URN: URN:SI:UM:
Pages: str. 419-426
Volume: ǂVol. ǂ252
Issue: ǂiss. ǂ3
Chronology: 2008
DOI: 10.1016/j.jtbi.2007.11.006
ID: 8724026