Spatio-temporal modelling explains the effect of reduced plasma membrane Ca[sup]2+ efflux on intracellular Ca[sup]2+ oscillations in hepatocytes

Marko Marhl (Avtor), Marko Gosak (Avtor), Matjaž Perc (Avtor), C. Jane Dixon (Avtor), Anne K. Green (Avtor)

Povzetek

In many non-excitable eukaryotic cells, including hepatocytes, ▫$Ca^{2+}$▫ oscillations play a key role in intra- and intercellular signalling, thus regulating many cellular processes from fertilisation to death. Therefore, understanding the mechanisms underlying these oscillations, and consequently understanding how they may be regulated, is of great interest. In this paper, we study the influence of reduced ▫$Ca^{2+}$▫ plasma membrane efflux on ▫$Ca^{2+}$▫ oscillations in hepatocytes. Our previous experiments with carboxyeosin show that a reduced plasma membrane ▫$Ca^{2+}$▫ efflux increases the frequency of ▫$Ca^{2+}$▫ oscillations, but does not affect the duration of individual transients. This phenomenon can be best explained by taking into account not only the temporal,but also the spatial dynamics underlying the generation of ▫$Ca^{2+}$▫ oscillations in the cell. Here we divide the cell into a grid of elements and treat the ▫$Ca^{2+}$▫ dynamics as a spatio-temporal phenomenon. By converting an existing temporal model into a spatio-temporal one, we obtain theoretical predictions that are in much better agreement with the experimental observations.

Ključne besede

celično signaliziranje;kalcijeve oscilacije;celične oscilacije;prostorsko-časovna dinamika;hepatociti;stohastične simulacije;cellular signalling;calcium oscillations;intracellular oscilations;spatio-temporal dynamics;hepatocytes;stochastic simulations;

Podatki

Jezik:	Angleški jezik
Leto izida:	2008
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FNM - Fakulteta za naravoslovje in matematiko
UDK:	519.83:53
COBISS:	16014344
ISSN:	0022-5193
Št. ogledov:	1159
Št. prenosov:	34
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarne ključne besede:	celično signaliziranje;kalcijeve oscilacije;celične oscilacije;prostorsko-časovna dinamika;hepatociti;stohastične simulacije;
URN:	URN:SI:UM:
Strani:	str. 419-426
Letnik:	ǂVol. ǂ252
Zvezek:	ǂiss. ǂ3
Čas izdaje:	2008
DOI:	10.1016/j.jtbi.2007.11.006
ID:	8724026