versatile fluorescent probes with remarkably large Stokes shift
Povzetek
A series of thirteen 2-acyl-6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole derivatives were synthesized by using a copper-catalyzed azomethine-imine-alkyne cycloaddition as a key step. These bimane-related fluorescent compounds with λ$_{ex}$ = 338–400 nm and λ$_{em}$ = 522–644 nm exhibit interesting and promising optical properties, including a remarkably large Stokes shift (150–268 nm), positive solvatochromism, and strong dependence of emission intensity on pH in a range of pH 2–10. The benzotriazolide- and N-succinimidyl ester-functionalized compounds were successfully used as fluorescent probes for protein labelling.
Ključne besede
6,7-dihidro-1H,5H-pirazolo[1,2-a]pirazoli;benzotriazolidi;fluorescentno označevanje;biokonjugacija;solvatokromija;6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole s;benzotriazolides;fluorescent imaging;bioconjugation;solvatochromism;
Podatki
Jezik: |
Angleški jezik |
Leto izida: |
2022 |
Tipologija: |
1.01 - Izvirni znanstveni članek |
Organizacija: |
UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo |
UDK: |
547.77 |
COBISS: |
100346115
|
ISSN: |
0143-7208 |
Št. ogledov: |
102 |
Št. prenosov: |
47 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Slovenski jezik |
Sekundarne ključne besede: |
6;7-dihidro-1H;5H-pirazolo[1;2-a]pirazoli;benzotriazolidi;fluorescentno označevanje;biokonjugacija;solvatokromija; |
Vrsta dela (COBISS): |
Članek v reviji |
Strani: |
str. 1-13 |
Zvezek: |
ǂVol. ǂ201 |
Čas izdaje: |
May 2022 |
DOI: |
10.1016/j.dyepig.2022.110224 |
ID: |
15721003 |