versatile fluorescent probes with remarkably large Stokes shift
Nejc Petek (Avtor), Bibi Erjavec (Avtor), Dejan Slapšak (Avtor), Aljaž Gaber (Avtor), Uroš Grošelj (Avtor), Franc Požgan (Avtor), Sebastijan Ričko (Avtor), Bogdan Štefane (Avtor), Marina Klemenčič (Avtor), Jurij Svete (Avtor)

Povzetek

A series of thirteen 2-acyl-6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole derivatives were synthesized by using a copper-catalyzed azomethine-imine-alkyne cycloaddition as a key step. These bimane-related fluorescent compounds with λ$_{ex}$ = 338–400 nm and λ$_{em}$ = 522–644 nm exhibit interesting and promising optical properties, including a remarkably large Stokes shift (150–268 nm), positive solvatochromism, and strong dependence of emission intensity on pH in a range of pH 2–10. The benzotriazolide- and N-succinimidyl ester-functionalized compounds were successfully used as fluorescent probes for protein labelling.

Ključne besede

6,7-dihidro-1H,5H-pirazolo[1,2-a]pirazoli;benzotriazolidi;fluorescentno označevanje;biokonjugacija;solvatokromija;6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole s;benzotriazolides;fluorescent imaging;bioconjugation;solvatochromism;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 547.77
COBISS: 100346115 Povezava se bo odprla v novem oknu
ISSN: 0143-7208
Št. ogledov: 102
Št. prenosov: 47
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: 6;7-dihidro-1H;5H-pirazolo[1;2-a]pirazoli;benzotriazolidi;fluorescentno označevanje;biokonjugacija;solvatokromija;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-13
Zvezek: ǂVol. ǂ201
Čas izdaje: May 2022
DOI: 10.1016/j.dyepig.2022.110224
ID: 15721003