Efficiency of four sampling methods used to detect two common nosocomial pathogens on textiles

Urška Rabuza (Avtor), Sonja Šostar-Turk (Avtor), Sabina Fijan (Avtor)

Povzetek

Detecting microorganisms on textiles is useful for many purposes, for example to determine the bioburden before laundering, assess the reduction in bacterial counts in connection with various laundry processes, or trace transfer routes in infection control investigations. Therefore a validated, reproducible and rational method is needed. For sampling microorganisms on textile surfaces the most commonly used method is the contact plate method using RODAC plates, first described by Hall and Hartnett followed by the swab sampling technique. Both methods can only capture microorganisms on the surface of the textiles while microorganisms that have penetrated into the deeper structure of the material will not be detected. In our research the contact plate method and the swabbing technique were compared with two wash-off methods. For the first wash-off method the destructive elution methodwas used, where microorganisms were eluted from the fabrics by shaking the fabrics for a certain time in an elution medium. For the fourth sampling method a nondestructive method that included a compact test device called MorapexŽ was used, which is based on forced desorption by pressing the microorganisms through the fabric without destroying the fabric. In our research, two types of microorganisms were included (Klebsiella pneumoniae andStaphylococcus aureus) that cause common nosocomial infections. The aim of this study was to compare the efficiency of the four sampling methods for detecting microorganisms on textiles and to determine the lowest concentration, which can still be detected. The percentage of microorganisms that were detected by both elution methods was substantially higher than by sampling of fabrics with the contact plate method or swabbing. It can be concluded that a nondestructive method using a modified MorapexŽ device can beapplied for quick determination of the hygienic condition of textiles.

Ključne besede

textile hygiene;Staphylococcus aureus;Klebsiella pneumoniae;swabbing;RODAC plates;elution method;Morapex;

Podatki

Jezik:	Angleški jezik
Leto izida:	2012
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FZV - Fakulteta za zdravstvene vede
UDK:	677:613
COBISS:	1822372
ISSN:	1746-7748
Št. ogledov:	1145
Št. prenosov:	98
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Neznan jezik
URN:	URN:SI:UM:
Vrsta dela (COBISS):	Delo ni kategorizirano
Strani:	str. 2099-2105
Letnik:	ǂVol. ǂ82
Zvezek:	ǂno. ǂ20
Čas izdaje:	2012
DOI:	10.1177/0040517512445339
ID:	1030085