Maruša Horvat (Avtor), Archana Pannuri (Avtor), Tony Romeo (Avtor), Iztok Dogša (Avtor), David Stopar (Avtor)

Povzetek

How viscoelastic properties of the extracellular matrix affect the various biological functions conferred by biofilms is an important question in microbiology. In this study viscoelastic response of Escherichia coli biofilms to genetically altered expression of extracellular matrix components was studied. Biofilms of the wild type E. coli MG1655 and its mutant strains producing different amounts of extracellular matrix components (curli, colonic acid, poly-β-1,6-N-acetyl-D-glucosamine) were used to examine the viscoelastic behavior of biofilms grown at solid -atmosphere interface. The results suggest that the presence of curli proteins dominates biofilm mechanical behavior. The rheological data indicate that cohesive energy of the biofilm was highest in the wild type strain. The results demonstrate the importance of extracellular matrix composition for biofilm mechanical properties. We propose that by genetically altering the expression of extracellular matrix polymers bacteria are able to modulate the mechanical properties of their local environment in accordance with bulk environmental conditions.

Ključne besede

Escherichia coli;biofilmi;reološke lastnosti;viskoelastičnost;zunajcelični polimeri;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579.24/.26:579.842:544
COBISS: 5060728 Povezava se bo odprla v novem oknu
ISSN: 1744-683X
Št. ogledov: 670
Št. prenosov: 456
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: biofilmi;Escherichia coli;zunajcelični polimeri;reološke lastnosti;viskoelastičnost;
Vrsta dela (COBISS): Znanstveno delo
Konec prepovedi (OpenAIRE): 2020-05-28
Strani: str. 5042-5051
Zvezek: ǂVol. ǂ15
Čas izdaje: 2019
DOI: 10.1039/c9sm00297a
ID: 11255017