Anja Klančnik (Avtor), Katarina Šimunović (Avtor), Jasna Kovač (Avtor), Orhan Sahin (Avtor), Zuowei Wu (Avtor), Darinka Vučković (Avtor), Maja Abram (Avtor), Qijing Zhang (Avtor), Sonja Smole Možina (Avtor)

Povzetek

We investigated the anti-Campylobacter activity of pinocembrin and its mechanism of action, as well as Campylobacter responses to pinocembrin treatment at the genetic and phenotypic levels, using C. jejuni NCTC 11168 and a multidrug efflux system repressor mutant (11168∆cmeR). At its minimal inhibitory concentration, pinocembrin significantly increased cell membrane permeability of Campylobacter. Interestingly, at sub-inhibitory concentrations, pinocembrin did not significantly alter membrane functionality and it increased bacterial fitness. Treatment with pinocembrin evoked decreased expression of ribosomal proteins and down-regulation of several NADH dehydrogenase I chain subunits and proteins involved in iron uptake. This suggests altered protein production and redox cycle and iron metabolism. Interestingly, the chelation of Fe ions during the treatment with pinocembrin increased C. jejuni survival, although there was no increase in the formation of reactive oxygen species. Pre-treatment of C. jejuni with sub-inhibitory concentrations of pinocembrin for 2 h resulted in a 1 log decrease in C. jejuni colony forming units in mice liver at 8 days post-infection, compared to untreated C. jejuni. These findings suggest that pinocembrin modulates the metabolic activity of C. jejuni and that pre-treatment of C. jejuni with pinocembrin influences its virulence potential in mice. This anti-Campylobacter potential of pinocembrin warrants further investigation.

Ključne besede

Campylobacter jejuni;pinocembrin;protimikrobne snovi;protimikrobna aktivnost;patogeni mikroorganizmi;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579.24:615.33
COBISS: 5133688 Povezava se bo odprla v novem oknu
ISSN: 2076-2607
Št. ogledov: 179
Št. prenosov: 61
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Campylobacter jejuni;pinocembrin;protimikrobne snovi;protimikrobna aktivnost;patogeni mikroorganizmi;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-15
Letnik: ǂVol. ǂ7
Zvezek: ǂiss. ǂ12
Čas izdaje: 2019
DOI: 10.3390/microorganisms7120675
ID: 13870644