Rok Tomšič (Avtor), David John Heath (Avtor), Ester Heath (Avtor), Jernej Markelj (Avtor), Andreja Kandolf (Avtor), Helena Prosen (Avtor)

Povzetek

In this study, a method was developed for the determination of five neonicotinoid pesticides (acetamiprid, clothianidin, imidacloprid, thiacloprid, and thiamethoxam) in propolis. Two sample preparation methods were tested: solid-phase extraction and the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method. The identities of analytes were confirmed using liquid chromatography–tandem mass spectrometry (LC-MS/MS) in the selected reaction monitoring mode. Solid-phase extraction resulted in cleaner extracts; therefore, the SPE-LC-MS/MS method was validated according to the SANTE protocol in triplicate at two spiking levels (10 ng/g and 50 ng/g). The average recoveries of analytes ranged from 61% to 101%, except for clothianidin (10–20%). The LOD ranged from 0.2 ng/g to 4.4 ng/g, whereas the LOQ was in the range of 0.8 ng/g–14.7 ng/g. In order to compensate for the matrix effect, matrix-matched calibration was used. Good accuracy (relative error: 1.9–10.4%) and good linearity (R$^2$ > 0.991) were obtained for all compounds. The optimised method was applied to 30 samples: 18 raw propolis and 12 ethanol tinctures. Acetamiprid, imidacloprid, and thiacloprid were detectable in seven samples but were still below the LOQ. This study is the first to report the determination of several neonicotinoid residues in propolis.

Ključne besede

neonikotinoidi;propolis;SPE;QuEChERS;LC-MS/MS;neonicotinoids;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 543.544.5:543.51
COBISS: 42430723 Povezava se bo odprla v novem oknu
ISSN: 1420-3049
Št. ogledov: 198
Št. prenosov: 109
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: neonikotinoidi;propolis;SPE;QuEChERS;LC-MS/MS;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-12
Letnik: ǂVol. ǂ25
Zvezek: ǂVol. ǂ24
Čas izdaje: Dec. 2020
DOI: 10.3390/molecules25245870
ID: 14354383