doktorska disertacija
Kristijan Skok (Avtor), Iztok Takač (Mentor), Uroš Maver (Komentor), Rajko Kavalar (Komentor)

Povzetek

Rak dojk je najpogostejši rak na svetu. Eden izmed njegovih podtipov je trojno negativni rak dojk (TNRD), za katerega je značilen agresiven potek, slabša prognoza in heterogenost. V svoji doktorski nalogi predstavim novo izolirano celično linijo TNRD, poimenovano MFUM-BrTNBC1. Linija je bila celovito karakterizirana (morfološko, fenotipsko, genotipsko). Za kontrole so služile komercialne celične linije z različnimi receptorskimi profili (MCF-7, MDA-MB-231, MDA-MB-453). Primerjalne morfološke analize so bile opravljene s pomočjo mikroskopije ter biomedicinske slikovne analize. S pomočjo imunocito/histokemičnih barvanj je bila preverjena izraženost hormonskih receptorjev (estrogen, progesteron), HER2, p53 in Ki-67 statusa. Ekspresija receptorjev je bila dodatno preverjena na transkripcijski ravni s qRT-PCR metodo. Dodatno je bilo opravljeno RNA sekvenciranje za molekularno analizo. Avtentičnost celičnih linij je bila preverjena s pomočjo STR profiliranja. MFUM-BrTNBC-1 celična linija je ohranila trojno negativni receptorski status in genetsko stabilnost do vsaj 6. pasaže. Rezultati STR analize in navzkrižna preverba z mednarodnimi bazami so dokazali avtentičnost celične linije. MFUM-BrTNBC1 se je po morfologiji celic razlikovala tudi od TNRD celične linije MDA-MB-231. Razlike od komercialne linije, predstavljajo tudi glavne prednostne lasnosti MFUM-BrTNBC-1. Te so izolacija iz primarnega tumorskega tkiva, popoln izvorni zapis, mednarodno poimenovanje, celoten in edinstven STR profil ter genetska stabilnost do vsaj 6. pasaže.

Ključne besede

trojno negativni rak dojk;MFUM-BrTNBC-1;rak dojk;in-vitro modeli;celične linije;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.08 - Doktorska disertacija
Organizacija: UM MF - Medicinska fakulteta
Založnik: K. Skok]
UDK: 576/577:602.3:618.19-006+616(043.3)
COBISS: 131347459 Povezava se bo odprla v novem oknu
Št. ogledov: 23
Št. prenosov: 1
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Analysis of phenotypical, molecular, and genetic properties of a novel triple-negative breast cancer cell line
Sekundarni povzetek: Breast cancer is the most common cancer worldwide. One of its subtypes is triple-negative breast cancer (TNBC), which is aggressive, has a worse prognosis, and is heterogeneous. In this thesis, I present a newly isolated TNBC cell line MFUM-BrTNBC-1. We performed a detailed characterization of morphological, phenotypic, and genotypic characteristics. As controls served 3 commercial breast cancer cell lines with different receptor profiles (MCF-7, MDA-MB-231, MDA-MB-453). Comparative morphological analysis was done using microscopy (light, fluorescence) and biomedical image analysis. Hormone receptor (estrogen, progesterone), p53 and Ki-67 statuses were determined by immunocyto/histochemical tests. Receptor expression was validated at the transcriptional level by qRT-PCR. RNA sequencing was utilised for additional analysis of molecular properties. Authenticity was checked by STR profiling. MFUM-BrTNBC-1 maintained the primary triple-negative receptor status and was genetically stable up to the 6th passage. STR profiles were crosschecked with international databases and confirmed authenticity. Morphologically, MFUM-BrTNBC-1 differed from the commercial TNBC cell line MDA-MB-231. The key differences and simultaneously advantages between MFUM-BrTNBC-1 and the commercial cell are, isolation from a primary tumour; good growth characteristics; identical phenotype to primary tissue; complete origin records, unique identifier; complete, unique STR profile and genetic stability up to (at least) 6th passage.
Sekundarne ključne besede: triple negative breast cancer;MFUM-BrTNBC-1;breast cancer;in vitro models;cell lines;
Vrsta dela (COBISS): Doktorska disertacija
Strani: IX, 105 f.
ID: 14934555