molecular characterisation, genetic stability, and comprehensive comparison with commercial breast cancer cell lines
Kristijan Skok (Avtor), Lidija Gradišnik (Avtor), Helena Sabina Čelešnik (Avtor), Marko Milojević (Avtor), Uroš Potočnik (Avtor), Gregor Jezernik (Avtor), Mario Gorenjak (Avtor), Monika Sobočan (Avtor), Iztok Takač (Avtor), Rajko Kavalar (Avtor), Uroš Maver (Avtor)

Povzetek

Triple-negative breast cancer (TNBC) is a breast cancer (BC) subtype that accounts for approximately 15–20% of all BC cases. Cancer cell lines (CLs) provide an efficient way to model the disease. We have recently isolated a patient-derived triple-negative BC CL MFUM-BrTNBC-1 and performed a detailed morphological and molecular characterisation and a comprehensive comparison with three commercial BC CLs (MCF-7, MDA-MB-231, MDA-MB-453). Light and fluorescence microscopy were used for morphological studies; immunocytochemical staining for hormone receptor, p53 and Ki67 status; RNA sequencing, qRT-PCR and STR analysis for molecular characterisation; and biomedical image analysis for comparative phenotypical analysis. The patient tissue-derived MFUMBrTNBC-1 maintained the primary triple-negative receptor status. STR analysis showed a stable and unique STR profile up to the 6th passage. MFUM-BrTNBC-1 expressed EMT transition markers and displayed changes in several cancer-related pathways (MAPK, Wnt and PI3K signalling; nucleotide excision repair; and SWI/SNF chromatin remodelling). Morphologically, MFUM-BrTNBC-1 differed from the commercial TNBC CL MDA-MB-231. The advantages of MFUM-BrTNBC-1 are its isolation from a primary tumour, rather than a metastatic site; good growth characteristics; phenotype identical to primary tissue; complete records of origin; a unique identifier; complete, unique STR profile; quantifiable morphological properties; and genetic stability up to (at least) the 6th passage.

Ključne besede

hormonal receptors;MFUM-BrTNBC-1;MCF-7;MDA-MB-231;MDA-MB-453;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM MF - Medicinska fakulteta
Založnik: MDPI AG
UDK: 618.19-006
COBISS: 91552259 Povezava se bo odprla v novem oknu
ISSN: 2073-4409
Št. ogledov: 0
Št. prenosov: 3
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Cell line;Hormones;Triple negative breast neoplasms;
Vrsta dela (COBISS): Članek v reviji
Strani: str. [1]-23
Letnik: ǂVol. ǂ11
Zvezek: ǂissue ǂ1
Čas izdaje: 2022
DOI: 10.3390/cells11010117
ID: 26197410