Electroporation with nanosecond pulses and bleomycin or cisplatin results in efficient cell kill and low metal release from electrodes

Angelika Vižintin (Avtor), Stefan Marković (Avtor), Janez Ščančar (Avtor), Damijan Miklavčič (Avtor)

Povzetek

Nanosecond electric pulses have several potential advantages in electroporation-based procedures over the conventional micro- and millisecond pulses including low level of heating, reduced electrochemical reactions and reduced muscle contractions making them alluring for use in biomedicine and food industry. The aim of this study was to evaluate if nanosecond pulses can enhance the cytotoxicity of chemotherapeutics bleomycin and cisplatin in vitro and to quantify metal release from electrodes in comparison to 100 μs pulses commonly used in electrochemotherapy. The effects of nanosecond pulse parameters (voltage, pulse duration, number of pulses) on cell membrane permeabilization, resealing and on cell survival after electroporation only and after electrochemotherapy with bleomycin and cisplatin were evaluated on Chinese hamster ovary cells. Application of permeabilizing nanosecond pulses in combination with chemotherapeutics resulted in successful cell kill. Higher extracellular concentrations of bleomycin – but not cisplatin – were needed to achieve the same decrease in cell survival with nanosecond pulses as with eight 100 μs pulses, however, the tested bleomycin concentrations were still considerably lower compared to doses used in clinical practice. Decreasing the pulse duration from microseconds to nanoseconds and concomitantly increasing the amplitude to achieve the same biological effect resulted in reduced release of aluminum ions from electroporation cuvettes.

Ključne besede

elektroporacija;nanosekundni pulzi;elektrokemoterapija;sproščanje kovin;electroporation;nanosecond pulses;electrochemotherapy;metal release;

Podatki

Jezik:	Angleški jezik
Leto izida:	2021
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL FE - Fakulteta za elektrotehniko
UDK:	602.621:616-006
COBISS:	57144835
ISSN:	1567-5394
Št. ogledov:	62
Št. prenosov:	28
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	elektroporacija;nanosekundni pulzi;elektrokemoterapija;sproščanje kovin;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-12
Letnik:	ǂVol. ǂ140
Zvezek:	107798
Čas izdaje:	Aug. 2021
DOI:	10.1016/j.bioelechem.2021.107798
ID:	16120573