Tamara Polajžer ... [et al.]
Tamara Polajžer (Avtor), Wencheng Peng (Avtor), Chenguo Yao (Avtor), Damijan Miklavčič (Avtor)

Povzetek

With the introduction of nanosecond (ns) pulses, it was suggested that such pulses could be used to permeabilize intracellular membranes, including the mitochondrial membrane. The results presented thus far, however, are not conclusive. Interestingly, the effect of longer microsecond (▫$\mu$▫s) pulses on changes in mitochondria has never been investigated. We, therefore, investigated the changes in mitochondrial membrane permeability through changes in mitochondrial membrane potential (MMP) in CHO and H9c2 cells after electroporation with 4 ns, 200 ns, and 100 ▫$\mu$▫s pulses. In the range of reversible electroporation, the decrease in MMP generally depended on the cell line. In CHO, ns pulses decreased MMP at lower electroporation intensities than μs. In H9c2, ns and ▫$\mu$▫s were equally effective. In the range of irreversible electroporation, MMP decreased even further, regardless of pulse duration and cell type. The analysis at different time points showed that the changes in MMP within the first hour after pulse treatment are dynamic. Our results on the efficacy of ns pulses are consistent with published data, but with this study we show that ▫$\mu$▫s pulses cause similar changes in MMP as ns pulses, demonstrating that electroporation affects MMP regardless of pulse duration. At the same time, however, differences in MMP changes were observed between different cell lines, indicating some dependence of MMP changes on cell type.

Ključne besede

elektroporacija;nanosekundni pulzi;mikrosekundni pulzi;mitohondriji;mitohondrijski membranski potencial;electroporation;nanosecond pulses;microsecond pulses;mitochondria;mitochondrial membranepotential;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FE - Fakulteta za elektrotehniko
Založnik: Mary Ann Liebert
UDK: 602.621
COBISS: 200294659 Povezava se bo odprla v novem oknu
ISSN: 2576-3113
Št. ogledov: 33
Št. prenosov: 3
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: elektroporacija;nanosekundni pulzi;mikrosekundni pulzi;mitohondriji;mitohondrijski membranski potencial;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 97-107
Letnik: ǂVol. ǂ6
Zvezek: ǂiss. ǂ2
Čas izdaje: Jun. 2024
DOI: 10.1089/bioe.2024.0007
ID: 24798321