Dina Ramić (Avtor), Anja Klančnik (Avtor), Sonja Smole Možina (Avtor), Iztok Dogša (Avtor)

Povzetek

The food-borne pathogen Campylobacter jejuni produces autoinducer-2 (AI-2) as an interspecies signalling molecule. AI-2 can trigger enhanced colonisation and biofilm formation, and this poses a serious risk to public health. To date, this communication system of C. jejuni is only partially understood, as detection and quantifi- cation of such autoinducer signalling molecules in complex media is hard to achieve. We have developed a whole-cell Vibrio harveyi-based biosensor assay to accurately quantify and follow production of AI-2 by C. jejuni 81–176 in a defined growth medium and in a model food system. Several V. harveyi strains were tested, but the most sensitive bioluminescent response to C. jejuni AI-2 was achieved with V. harveyi MM30, likely due to its ability to self-amplify the response to AI-2. The AI-2 concentrations measured by this biosensor were confirmed using an independent analytical method, HPLC-FLD, which we introduced for Campylobacter analytics for the first time. The AI-2 concentration produced by C. jejuni 81–176 in the model food system was ~5-fold that in the defined growth medium, at the same cell density. Together with the linear increments in AI-2 concentrations with cell density, this suggests that in C. jejuni, AI-2 represents a metabolic by-product rather than a true quorum- sensing molecule. This biosensor method is highly sensitive, as shown by the reduction in the limit of detection (by a factor of 100) compared to HPLC-FLD, and it enables quantification of AI-2 in complex matrices, such as food, which will help to improve the quality and safety of food production.

Ključne besede

patogeni mikroorganizmi;Campylobacter jejuni;medcelično signaliziranje;biosenzorji;kvantifikacija;varnost živil;AI-2 quantification;biosensor method;HPLC-FLD;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579.24/.26:53.082.9
COBISS: 110249219 Povezava se bo odprla v novem oknu
ISSN: 0956-5663
Št. ogledov: 27
Št. prenosov: 10
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: patogeni mikroorganizmi;Campylobacter jejuni;medcelično signaliziranje;biosenzorji;kvantifikacija;varnost živil;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-8
Zvezek: ǂVol. ǂ212
Čas izdaje: 2022
DOI: 10.1016/j.bios.2022.114439
ID: 16372737