Alessandro Alan Porporati (Avtor), Yvonne Mödinger (Avtor), Sarah Fischer (Avtor), Sara Polajžer (Avtor), Melanie Mettang (Avtor), Ulrike Deisinger (Avtor), Matejka Podlogar (Avtor), Rihard Trebše (Avtor), Nika Lovšin (Avtor)

Povzetek

Ten percent of patients undergoing total hip arthroplasty (THA) require revision surgery. One of the reasons for THA are wear particles released from the implants that can activate the immune defense and cause osteolysis and failure of the joint implant. The discrepancies between reports on toxicity and immunogenicity of the implant materials led us to this study in which we compared toxicity and immunogenicity of well-defined nanoparticles from Al2O3, zirconia-toughened alumina (ZTA), and cobalt chrome (CoCr), a human THP-1 macrophage cell line, human PBMCs, and therefrom-derived primary macrophages. None of the tested materials decreased the viability of THP-1 macrophages nor human primary macrophages at the 24 h time point, indicating that at concentrations from 0.05 to 50 µm3/cell the tested materials are non-toxic. Forty-eight hours of treatment of THP-1 macrophages with 5 µm3/cell of CoCr and Al2O3 caused 8.3-fold and 4.6-fold increases in TNF-α excretion, respectively, which was not observed for ZTA. The comparison between THP-1 macrophages and human primary macrophages revealed that THP-1 macrophages show higher activation of cytokine expression in the presence of CoCr and Al2O3 particles than primary macrophages. Our results indicate that ZTA is a non-toxic implant material with no immunogenic effects in vitro.

Ključne besede

orthopaedic implant;alumina;zirconia-toughened alumina (ZTA);nanoparticles;macrophages;cell viability;inflammation;cytokine secretion;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FFA - Fakulteta za farmacijo
UDK: 66.017-022.513.2:620.3
COBISS: 147226627 Povezava se bo odprla v novem oknu
ISSN: 1422-0067
Št. ogledov: 37
Št. prenosov: 24
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: ortopedski vsadek;aluminijev oksid;utrjen s cirkonijem (ZTA);nanodelci;makrofagi;sposobnost preživetja celic;vnetje;izločanje citokinov;Citokini;Nanomateriali;Vnetje;
Vrsta dela (COBISS): Članek v reviji
Strani: 12 str.
Letnik: ǂVol. ǂ24, art. 6482
Zvezek: ǂiss. ǂ7
Čas izdaje: 2023
DOI: 10.3390/ijms24076482
ID: 18434265