CRISPR technologies in Chinese hamster ovary cell line engineering

Katja Glinšek (Avtor), Krištof Bozovičar (Avtor), Tomaž Bratkovič (Avtor)

Povzetek

The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and enhanced product quality. This review summarizes recent advances in the application of CRISPR technology for CHO cell line engineering with a particular focus on glycosylation modulation, productivity enhancement, tackling adventitious agents, elimination of problematic host cell proteins, development of antibiotic-free selection systems, site-specific transgene integration, and CRISPR-mediated gene activation and repression. The review highlights the potential of CRISPR technology in CHO cell line genome editing and epigenetic engineering for the more efficient and cost-effective development of biopharmaceuticals while ensuring the safety and quality of the final product.

Ključne besede

Chinese hamster ovary cells;cell line development;CRISPR-Cas;genome editing;glycoengineering;augmenting productivity;

Podatki

Jezik:	Angleški jezik
Leto izida:	2023
Tipologija:	1.02 - Pregledni znanstveni članek
Organizacija:	UL FFA - Fakulteta za farmacijo
UDK:	575.111
COBISS:	150745347
ISSN:	1422-0067
Št. ogledov:	35
Št. prenosov:	5
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	jajčne celice kitajskega hrčka;razvoj celične linije;urejanje genoma;glikoinženiring;enocelično zaporedje;povečanje produktivnosti;Genomika;
Vrsta dela (COBISS):	Članek v reviji
Strani:	26 str.
Letnik:	ǂVol. ǂ24
Zvezek:	ǂiss. ǂ9, ǂart. ǂ8144
Čas izdaje:	2023
DOI:	10.3390/ijms24098144
ID:	22635365