ǂa ǂdual-function cysteine protease with endo- and transpeptidase activity
Katarina Petra van Midden (Avtor), Melissa Mantz (Avtor), Marko Fonović (Avtor), Martin Gazvoda (Avtor), Jurij Svete (Avtor), Pitter F. Huesgen (Avtor), Renier A. L. van der Hoorn (Avtor), Marina Klemenčič (Avtor)

Povzetek

Proteases, essential regulators of plant stress responses, remain enigmatic in their precise functional roles. By employing activity-based probes for real-time monitoring, this study aimed to delve into protease activities in Chlamydomonas reinhardtii exposed to oxidative stress induced by hydrogen peroxide. However, our work revealed that the activity-based probes strongly labelled three non-proteolytic proteins—PsbO, PsbP, and PsbQ—integral components of photosystem II's oxygen-evolving complex. Subsequent biochemical assays and mass spectrometry experiments revealed the involvement of CrCEP1, a previously uncharacterized papain-like cysteine protease, as the catalyst of this labelling reaction. Further experiments with recombinant CrCEP1 and PsbO proteins replicated the reaction in vitro. Our data unveiled that endopeptidase CrCEP1 also has transpeptidase activity, ligating probes and peptides to the N-termini of Psb proteins, thereby expanding the repertoire of its enzymatic activities. The hitherto unknown transpeptidase activity of CrCEP1, working in conjunction with its proteolytic activity, unveils putative complex and versatile roles for proteases in cellular processes during stress responses.

Ključne besede

proteoliza;sonde na osnovi aktivnosti;papainu podobna cisteinska proteaza;transpeptidacija;zelene alge;proteolysis;activity-based probes;papain-like cysteine protease;transpeptidation;green algae;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 577.15
COBISS: 197196291 Povezava se bo odprla v novem oknu
ISSN: 0141-8130
Št. ogledov: 62
Št. prenosov: 17
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: proteoliza;sonde na osnovi aktivnosti;papainu podobna cisteinska proteaza;transpeptidacija;zelene alge;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-15
Letnik: ǂVol. ǂ271
Zvezek: ǂpt. ǂ1, [article no.] 132505
Čas izdaje: Jun. 2024
DOI: 10.1016/j.ijbiomac.2024.132505
ID: 24230211