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Optimizing CRISPR–Cas9-mediated TCR knockout in primary mouse CD8+, CD4+, and regulatory T cells
Andrea Šarac, Klemen Kunej, Kristina Manzoni, Karen Butina, Katja Leben, Simon Horvat, Jelka Pohar, Anže Smole
Izvirni znanstveni članek
Oznake: CRISPR-Cas9;imunologija;celice;genome editing;CRISPR–Cas9 RNP;trac knockout;primary mouse T cells;regulatory T cells;electroporation;
CRISPR–Cas9 genome editing has transformed biomedical research by enabling precise and efficient genetic modification. Among its applications, gene knockout using ribonucleoprotein (RNP) complexes provides a non-viral strategy for introducing loss-of-function mutations. Here, we present an optimized ...
Leto: 2026 Vir: Repozitorij Univerze v Ljubljani (RUL)
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