Št. zadetkov: 31
Poljudni članek
Oznake:
čebele;gensko spremenjeni organizmi;
Navzočnost in določanje gensko spremenjenih organizmov
Leto:
2012
Vir:
Digitalni repozitorij raziskovalnih organizacij Slovenije
Izvirni znanstveni članek
Oznake:
molecular diagnostics;direct quantification;viruses;virus reference materials;human cytomegalovirus;digital PCR;
DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly affect the final measurements due to variable DNA yields and removal of inhibitors, which leads to increased inter-laboratory variability of qPCR ...
Leto:
2016
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
real-time PCR;molecular diagnostics;human cytomegalovirus;DNA quantification;digital PCR;
Digital PCR (dPCR) is beginning to supersede real-time PCR (qPCR) for quantification of nucleic acids in many different applications. Several analytical properties of the two most commonly used dPCR platforms, namely the QX100 system (Bio-Rad) and the 12.765 array of the Biomark system (Fluidigm), h ...
Leto:
2016
Vir:
Nacionalni inštitut za biologijo (NIB)
Končno poročilo o rezultatih raziskav
Oznake:
Key comparison CCQM-K86 was performed to demonstrate and document the capacity of interested National Metrology Institutes (NMIs) and Designated Institutes (DIs) in the determination of the relative quantity of two specific genomic DNA fragments present in a biological tissue.
The study provides th ...
Leto:
2012
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
class III peroxidases;potato virus YNTN;Solanum tuberosum;
Peroxidases in the early responses of different potato cultivars to infection by potato virus YNTN
Leto:
2008
Vir:
Digitalni repozitorij raziskovalnih organizacij Slovenije
Magistrsko delo
Oznake:
RT-qPCR;RT-dPCR;virusi;genom virusa;magistrska dela;
Izbira pravilne diagnostične metode je ključna za zagotavljanje natančnih in zanesljivih rezultatov pri odkrivanju virusov. To predstavlja poseben izziv pri RNA-virusih, kot je SARS-CoV-2, saj je v tem primeru najprej treba izvesti reverzno transkripcijo (RT), kar lahko pomembno vpliva na rezultate ...
Leto:
2025
Vir:
Fakulteta za kemijo in kemijsko tehnologijo (UL FKKT)
Izvirni znanstveni članek
Oznake:
digital PCR;diagnostics;
Background
Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being used to diagnose tuberculosis (TB). While qualitative methods are adequate for diagnosis, the therapeutic monitoring of TB patients requires quantitative methods currently performed using smear microsco ...
Leto:
2016
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
nukleinske kisline;dPCR;metode ekstrakcije DNK;gram-negativne bakterije;nucleic acid;DNA extraction methods;Gram-negative bacteria;
Detection and quantification of DNA biomarkers relies heavily on the yield and quality of DNA obtained by extraction from different matrices. Although a large number of studies have compared the yields of different extraction methods, the repeatability and intermediate precision of these methods hav ...
Leto:
2023
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
extraction methods;genetically modified organisms;GMO;decision- making system (biology);GMO testing;cetyltrimethylammonium bromide;CTAB;
Current methods based on DNA targets for the detection, identification and quantification of genetically modified organisms (GMOs) involve extraction of the DNA. Different extraction procedures have been developed for the great variety of samples from food, feed, seeds and particular plant parts. Th ...
Leto:
2015
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
droplet digital PCR;ddPCR;genetically modified organisms;
In this study, the applicability of droplet digital PCR (ddPCR) for routine analysis in food and feed samples was demonstrated with the quantification of genetically modified organisms (GMOs). Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analys ...
Leto:
2013
Vir:
Nacionalni inštitut za biologijo (NIB)