Št. zadetkov: 28
Poljudni članek
Oznake:
čebele;gensko spremenjeni organizmi;
Navzočnost in določanje gensko spremenjenih organizmov
Leto:
2012
Vir:
Digitalni repozitorij raziskovalnih organizacij Slovenije
Izvirni znanstveni članek
Oznake:
molecular diagnostics;direct quantification;viruses;virus reference materials;human cytomegalovirus;digital PCR;
DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly affect the final measurements due to variable DNA yields and removal of inhibitors, which leads to increased inter-laboratory variability of qPCR ...
Leto:
2016
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
real-time PCR;molecular diagnostics;human cytomegalovirus;DNA quantification;digital PCR;
Digital PCR (dPCR) is beginning to supersede real-time PCR (qPCR) for quantification of nucleic acids in many different applications. Several analytical properties of the two most commonly used dPCR platforms, namely the QX100 system (Bio-Rad) and the 12.765 array of the Biomark system (Fluidigm), h ...
Leto:
2016
Vir:
Nacionalni inštitut za biologijo (NIB)
Končno poročilo o rezultatih raziskav
Oznake:
Key comparison CCQM-K86 was performed to demonstrate and document the capacity of interested National Metrology Institutes (NMIs) and Designated Institutes (DIs) in the determination of the relative quantity of two specific genomic DNA fragments present in a biological tissue.
The study provides th ...
Leto:
2012
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
class III peroxidases;potato virus YNTN;Solanum tuberosum;
Peroxidases in the early responses of different potato cultivars to infection by potato virus YNTN
Leto:
2008
Vir:
Digitalni repozitorij raziskovalnih organizacij Slovenije
Izvirni znanstveni članek
Oznake:
digital PCR;diagnostics;
Background
Real-time PCR (qPCR) based methods, such as the Xpert MTB/RIF, are increasingly being used to diagnose tuberculosis (TB). While qualitative methods are adequate for diagnosis, the therapeutic monitoring of TB patients requires quantitative methods currently performed using smear microsco ...
Leto:
2016
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
droplet digital PCR;ddPCR;genetically modified organisms;
Quantitative analysis of food and feed samples with droplet digital PCR
Leto:
2013
Vir:
Digitalni repozitorij raziskovalnih organizacij Slovenije
Izvirni znanstveni članek
Oznake:
nukleinske kisline;dPCR;metode ekstrakcije DNK;gram-negativne bakterije;nucleic acid;DNA extraction methods;Gram-negative bacteria;
Detection and quantification of DNA biomarkers relies heavily on the yield and quality of DNA obtained by extraction from different matrices. Although a large number of studies have compared the yields of different extraction methods, the repeatability and intermediate precision of these methods hav ...
Leto:
2023
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
extraction methods;genetically modified organisms;GMO;decision- making system (biology);GMO testing;cetyltrimethylammonium bromide;CTAB;
Current methods based on DNA targets for the detection, identification and quantification of genetically modified organisms (GMOs) involve extraction of the DNA. Different extraction procedures have been developed for the great variety of samples from food, feed, seeds and particular plant parts. Th ...
Leto:
2015
Vir:
Nacionalni inštitut za biologijo (NIB)
Izvirni znanstveni članek
Oznake:
genetically modified organisms;digital PCR;GMO quantification;complex matrices;
The increased use of genetically modified organisms (GMOs) is accompanied by increased complexity of the matrices that contain GMOs. The most common DNA-based approach for GMO detection and quantification is real-time quantitative polymerase chain reaction (qPCR). However, as qPCR is sensitive to in ...
Leto:
2019
Vir:
Nacionalni inštitut za biologijo (NIB)