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Št. zadetkov: 7
Testing of begomoviruses capable of infecting tomatoes and plants of the family Cucurbitaceae by PCR
Nataša Mehle, Jakob Brodarič, Zala Kogej, Tjaša Jakomin, Ana Vučurović
Končno poročilo o rezultatih raziskav
Oznake: diagnostika;validacija metod;begomoviruses;EURL-Virology;
Detection of begomoviruses capable of infecting tomatoes and plants of the family Cucurbitaceae.
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
Rapid loop-mediated isothermal amplification assays for grapevine yellows phytoplasmas on crude leaf-vein homogenate has the same performance as qPCR
Polona Kogovšek, Nataša Mehle, Anja Pugelj, Tjaša Jakomin, Hans-Josef Schroers, Maja Ravnikar, Marina Dermastia
Izvirni znanstveni članek
Oznake: real-time LAMP;grapevine yellows phytoplasma;validation;
A fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for ‘Candidatus Phytoplasama solani’ (Bois noir phytoplasma; BNp) detection was developed and optimised for rapid laboratory and on-site BNp detection. This assay is highly specific, rapid and as sensitive as qPCR. It ...
Leto: 2017 Vir: Nacionalni inštitut za biologijo (NIB)
Filling the gaps in diagnostics of Pepino mosaic virus and Potato spindle tuber viroid in water and tomato seeds and leaves
Nataša Mehle, Polona Kogovšek, Nejc Rački, Tjaša Jakomin, Ion Gutierrez-Aguirre, Petra Kramberger, Maja Ravnikar
Izvirni znanstveni članek
Oznake: PSTVd;PepMV;seeds;water;concentration;loop-mediated isothermal amplification;
Waterborne and seedborne Pepino mosaic virus (PepMV) and Potato spindle tuber viroid (PSTVd) pose serious threats to tomato production due to seed transmission and mechanical transmission, coupled with their long-term stability outside the host plant. Therefore, rapid and sensitive diagnostic proced ...
Leto: 2017 Vir: Nacionalni inštitut za biologijo (NIB)
Epidemiology of Flavescence dorée and hazelnut decline in Slovenia
Zala Kogej, Gabrijel Seljak, Tjaša Jakomin, Jakob Brodarič, Ana Vučurović, Sandra Pedemay, Pascal Salar, Sylvie Malembic-Maher, Xavier Foissac, Nataša Mehle
Izvirni znanstveni članek
Oznake: fitoplazme;leska;trta;zlata trsna rumenica;bolezni vinske trte;rastlinske bolezni;phytoplasmas;haselnuts;grapevine;Flavescence dorée;epidemiology;plant disease;
Flavescence dorée (FD) phytoplasma from 16SrV-C and -D subgroups cause severe damage to grapevines throughout Europe. This phytoplasma is transmitted from grapevine to grapevine by the sap-sucking leafhopper Scaphoideus titanus. European black alder and clematis serve as perennial plant reservoirs f ...
Leto: 2023 Vir: Nacionalni inštitut za biologijo (NIB)
Accurate quantification and characterization of adeno-associated viral vectors
David Dobnik, Polona Kogovšek, Tjaša Jakomin, Nejc Košir, Magda Tušek-Žnidarič, Maja Leskovec, Stephen M. Kaminsky, Janet Mostrom, Hyunmi Lee, Maja Ravnikar
Izvirni znanstveni članek
Oznake: absolute quantification;AAV;gene therapy;electron microscopy;digital PCR;real-time PCR;
One of the main challenges in the gene therapy viral vector development is to establish an optimized process for its large scale production. This requires optimization for upstream and downstream processes as well as methods that enable the step-by step analytical characterization of the virus, the ...
Leto: 2019 Vir: Nacionalni inštitut za biologijo (NIB)
In-depth comparison of adeno-associated virus containing fractions after CsCl ultracentrifugation gradient separation
Mojca Janc, Kaja Zevnik, Ana Dolinar, Tjaša Jakomin, Maja Štalekar, Katarina Bačnik, Denis Kutnjak, Magda Tušek-Žnidarič, L. Zentilin, Dmitri G. Fedorov, David Dobnik
Izvirni znanstveni članek
Oznake: rekombinantni z adenovirusi povezani virusi;centrifugiranje v CsCl gradientu;analitične metode;digitalni kapljični PCR;virologija;recombinant adeno-associated viruses (rAAVs);CsCl ultracentrifugation gradient;analytical methods;digital droplet PCR (ddPCR);transmission electron microscopy (TEM);analytical ultracentrifugation (AUC);size-exclusion chromatography coupled with multi-angle light scattering (SEC-MALS);Illumina sequencing;virology;
Recombinant adeno-associated viruses (rAAVs) play a pivotal role in the treatment of genetic diseases. However, current production and purification processes yield AAV-based preparations that often contain unwanted empty, partially filled or damaged viral particles and impurities, including residual ...
Leto: 2024 Vir: Nacionalni inštitut za biologijo (NIB)
List of tests for validation
Špela Alič, Géraldine Anthoine, A Chabirand, Anne-Marie Chappé, Tanja Dreo, Tjaša Jakomin, L Laurenson, Tadeja Lukežič, Nataša Mehle, E Metz-Verschure, H Mouaziz, J Oorspronk, Manca Pirc, Maja Ravnikar, Naomi te Braak, J. Tomlinson, Marcel Westenberg
Elaborat, predštudija, študija
Oznake: pests;tests;
We prepared a list of methods and tests for validation in test performance study (TPS) Round 1, both for laboratory and on-site use, for 6 selected pests: Erwinia amylovora, Pantoea stewartiisubsp. stewartii, citrus tristeza virus, plum pox virus, Fusarium circinatum and Bursaphelenchus xylophilus. ...
Leto: 2019 Vir: Nacionalni inštitut za biologijo (NIB)
Št. zadetkov: 7
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