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Št. zadetkov: 5
High-throughput sequencing detection and molecular characterization of viral diseases of grapevine (Vitis vinifera L.) and their elimination by thermotherapy and meristem tissue culture
Vanja Miljanić, Nataša Štajner
Doktorska disertacija
Oznake: grapevine;high-throughput sequencing;viral diseases;virome;diagnosis;genetic diversity;thermotherapy;micrographing;meristem tissue culture;
We studied the virome of preclonal candidates obtained after mass selection of grapevines using HTS technology. Nine viruses (GFLV, GLRaV-3, GRSPaV, GFkV, GSyV-1, GRVFV, GRGV, GPGV, and RBDV) and two viroids (HSVd and GYSVd-1) were identified. GRGV, GRVFV, and GSyV-1 were detected for the first time ...
Leto: 2022 Vir: Biotehniška fakulteta (UL BF)
Identifikacija virusov pri klonu vinske trte (Vitis vinifera L.) sorte malvazija ter njihova eliminacija s termoterapijo
Hana Flajnik, Nataša Štajner, Vanja Miljanić, Tjaša Lukan
Magistrsko delo
Oznake: vinska trta;virusi;eliminacija virusov;termoterapija;RT-PCR;RTqPCR;tkivna kultura vršičkov;RNAi;DCL;AGO;RDR;
Vinska trta Vitis vinifera L. je ena ekonomsko najpomembnejših kmetijskih rastlin. Zaradi vegetativnega razmnoževanja je nagnjena k okužbam z virusi in viroidi, ki vplivajo na njeno rast in pridelek. Z metodama RT-PCR in RT-qPCR smo detektirali sedem virusov od skupno 15 virusov, ki so vključeni v o ...
Leto: 2023 Vir: Biotehniška fakulteta (UL BF)
Elimination of eight viruses and two viroids from preclonal candidates of six grapevine varieties (Vitis vinifera L.) through In vivo thermotherapy and in vitro meristem tip micrografting
Vanja Miljanić, Denis Rusjan, Andreja Škvarč, Philippe Chatelet, Nataša Štajner
Izvirni znanstveni članek
Oznake: Vitis vinifera;grapevine;viruses;viroids;micrografting;thermotherapy;
Viruses and virus-like organisms are amajor problemin viticultureworldwide. They cannot be controlled by standard plant protection measures, and once infected, plants remain infected throughout their life; therefore, the propagation of healthy vegetative material is crucial. In vivo thermotherapy at ...
Leto: 2022 Vir: Biotehniška fakulteta (UL BF)
Small RNA sequencing and multiplex RT-PCR for diagnostics of grapevine viruses and virus-like organisms
Vanja Miljanić, Jernej Jakše, Denis Rusjan, Andreja Škvarč, Nataša Štajner
Izvirni znanstveni članek
Oznake: vitis vinifera;grapevine;virome;metagenomic analysis;viruses;sRNA-seq;molecular diagnostics;
Metagenomic approaches used for virus diagnostics allow for rapid and accurate detection of all viral pathogens in the plants. In order to investigate the occurrence of viruses and virus-like organisms infecting grapevine from the Ampelographic collection Kromberk in Slovenia, we used Ion Torrent sm ...
Leto: 2022 Vir: Biotehniška fakulteta (UL BF)
Virome status of preclonal candidates of grapevine varieties (Vitis vinifera L.) from the Slovenian wine-growing region Primorska as determined by high-throughput sequencing
Vanja Miljanić, Jernej Jakše, Urban Kunej, Denis Rusjan, Andreja Škvarč, Nataša Štajner
Izvirni znanstveni članek
Oznake: Vitis vinifera;preclonal candidates;HTS;viruses;viroids;
Diseases caused by viruses and virus-like organisms are one of the major problems in viticulture and grapevine marketing worldwide. Therefore, rapid and accurate diagnosis and identification is crucial. In this study, we used HTS of virus- and viroid-derived small RNAs to determine the virome status ...
Leto: 2022 Vir: Biotehniška fakulteta (UL BF)
Št. zadetkov: 5
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