Andrea Kos (Author), Bojan Papić (Author), Majda Golob (Author), Jana Avberšek (Author), Darja Kušar (Author), Tijana Ledina (Author), Jasna Đorđević (Author), Snežana Bulajić (Author)

Abstract

The potential risk to human and animal health provides a rationale for research on methicillin-resistant staphylococci (MRS) and mammaliicocci (MRM) in dairy herds. Here, we aimed to estimate their occurrence in the bulk tank milk (BTM) samples collected in 2019–2021 from 283 bovine dairy farms in the Belgrade district. We used whole-genome sequencing to characterize the obtained isolates and assess their genetic relatedness. A total of 70 MRS/MRM were recovered, most frequently Staphylococcus haemolyticus and Mammaliicoccus sciuri. Five clusters of 2–4 genetically related isolates were identified and epidemiological data indicated transmission through, e.g., farm visits by personnel or milk collection trucks. Most MRSA isolates belonged to the typical livestock-associated lineage ST398-t034. One MRSA isolate (ST152-t355) harbored the PVL-encoding genes. Since MRS/MRM isolates obtained in this study frequently harbored genes conferring multidrug resistance (MDR), this argues for their role as reservoirs for the spread of antimicrobial resistance genes. The pipeline milking system and total bacterial count >100,000 CFU/mL were significantly associated with higher occurrences of MRS/MRM. Our study confirms that BTM can be a zoonotic source of MRS, including MDR strains. This highlights the urgent need for good agricultural practices and the continuous monitoring of MRS/MRM in dairy farms.

Keywords

Staphylococcus aureus;milk;virulence factors;whole genome sequencing;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL VF - Veterinary Faculty
UDC: 636.09:616:577
COBISS: 167972099 Link will open in a new window
ISSN: 2079-6382
Views: 9
Downloads: 0
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Other data

Secondary keywords: Staphylococcus aureus;Milk;Virulence Factors;Whole Genome Sequencing;
Type (COBISS): Article
Pages: str. 1-19
Volume: ǂVol. ǂ10
Issue: ǂissue ǂ10, [art. no.] 1529
Chronology: 2023
DOI: 10.3390/antibiotics12101529
ID: 20202145