Sandra Janežič (Avtor), Mojca Potočnik (Avtor), Valerija Zidarič (Avtor), Maja Rupnik (Avtor)

Povzetek

Clostridium difficile is one of the most important human and animal pathogens. However, the bacterium is ubiquitous and can be isolated from various sources. Here we report the prevalence and characterization of C. difficile in less studied environmental samples, puddle water (n = 104) and soil (n = 79). C. difficile was detected in 14.4% of puddle water and in 36.7% of soil samples. Environmental strains displayed antimicrobial resistance patterns comparable to already published data of human and animal isolates. A total of 480 isolates were grouped into 34 different PCR ribotypes. More than half of these (52.9%; 18 of 34) were already described in humans or animals. However, 14 PCR ribotypes were new in our PCR ribotype library and all but one were non-toxigenic. The multilocus sequence analysis of these new PCR ribotypes revealed that non-toxigenic environmental isolates are phylogenetically distinct and belong to three highly divergent clades, two of which have not been described before. Our data suggest that environment is a potential reservoir of genetically diverse population of C. difficile.

Ključne besede

clostridium difficile;environment;isolation;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM MF - Medicinska fakulteta
UDK: 579.852.13
COBISS: 512659256 Povezava se bo odprla v novem oknu
ISSN: 1932-6203
Št. ogledov: 893
Št. prenosov: 334
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Clostridium difficile;
URN: URN:SI:UM:
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 1-12
Letnik: ǂVol. ǂ11
Zvezek: ǂiss. ǂ11
Čas izdaje: 2016
DOI: 10.1371/journal.pone.0167101
ID: 10843430