Hedwig Kurka (Avtor), Armin Ehrenreich (Avtor), Wolfgang Ludwig (Avtor), Marc Monot (Avtor), Maja Rupnik (Avtor), Frédéric Barbut (Avtor), Alexander Indra (Avtor), Bruno Dupuy (Avtor), Wolfgang Liebl (Avtor)

Povzetek

PCR-ribotyping is a broadly used method for the classification of isolates of Clostridium difficile, an emerging intestinal pathogen, causing infections with increased disease severity and incidence in several European and North American countries. We have now carried out clustering analysis with selected genes of numerous C. difficile strains as well as gene content comparisons of their genomes in order to broaden our view of the relatedness of strains assigned to different ribotypes. We analyzed the genomic content of 48 C. difficile strains representing 21 different ribotypes. The calculation of distance matrix-based dendrograms using the neighbor joining method for 14 conserved genes (standard phylogenetic marker genes) from the genomes of the C. difficile strains demonstrated that the genes from strains with the same ribotype generally clustered together. Further, certain ribotypes always clustered together and formed ribotype groups, i.e. ribotypes 078, 033 and 126, as well as ribotypes 002 and 017, indicating their relatedness. Comparisons of the gene contents of the genomes of ribotypes that clustered according to the conserved gene analysis revealed that the number of common genes of the ribotypes belonging to each of these three ribotype groups were very similar for the 078/033/126 group (at most 69 specific genes between the different strains with the same ribotype) but less similar for the 002/017 group (86 genes difference). It appears that the ribotype is indicative not only of a specific pattern of the amplified 16S23S rRNA intergenic spacer but also reflects specific differences in the nucleotide sequences of the conserved genes studied here. It can be anticipated that the sequence deviations of more genes of C. difficile strains are correlated with their PCR-ribotype. In conclusion, the results of this study corroborate and extend the concept of clonal C. difficile lineages, which correlate with ribotypes affiliation.

Ključne besede

clostridium difficile;comparative genomics;RNA polymerase;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM MF - Medicinska fakulteta
UDK: 579.852.13
COBISS: 512376632 Povezava se bo odprla v novem oknu
ISSN: 1932-6203
Št. ogledov: 944
Št. prenosov: 302
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Clostridium Difficile;
URN: URN:SI:UM:
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 1-11
Letnik: ǂVol. ǂ9
Zvezek: ǂiss. ǂ1
Čas izdaje: Jan. 2014
DOI: 10.1371/journal.pone.0086535
ID: 10843447