N Henning Zaiß (Avtor), Maja Rupnik (Avtor), Ed J Kuijper (Avtor), Celine Harmanus (Avtor), Dolf Michielsen (Avtor), Koen Janssens (Avtor), Ulrich Nübel (Avtor)

Povzetek

Background: Genotyping of epidemic Clostridium difficile strains is necessary to track their emergence and spread. Portability of genotyping data is desirable to facilitate inter-laboratory comparisons and epidemiological studies. Results: This report presents results from a systematic screen for variation in repetitive DNA in the genome of C. difficile. We describe two tandem repeat loci, designated 'TR6' and 'TR10', which display extensive sequence variation that may be useful for sequence-based strain typing. Based on an investigation of 154 C. difficile isolates comprising 75 ribotypes, tandem repeat sequencing demonstrated excellent concordance with widely used PCR ribotyping and equal discriminatory power. Moreover, tandem repeat sequences enabled the reconstruction of the isolates' largely clonal population structure and evolutionary history. Conclusion: We conclude that sequence analysis of the two repetitive loci introduced here may be highly useful for routine typing of C. difficile. Tandem repeat sequence typing resolves phylogenetic diversity to a level equivalent to PCR ribotypes. DNA sequences may be stored in databases accessible over the internet, obviating the need for the exchange of reference strains.

Ključne besede

clostridium difficile;mikrobiologija;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM MF - Medicinska fakulteta
UDK: 579.6
COBISS: 652063 Povezava se bo odprla v novem oknu
ISSN: 1471-2180
Št. ogledov: 945
Št. prenosov: 354
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: Clostridium difficile;mikrobiologija;tipizacija sevov;
URN: URN:SI:UM:
Vrsta dela (COBISS): Znanstveno delo
Strani: 11 str.
Letnik: ǂLetn. ǂ9
Zvezek: ǂšt. ǂ6
Čas izdaje: 2009
DOI: 10.1186/1471-2180-9-6
ID: 10845019