establishing links between animals and humans on livestock holdings
Jana Avberšek (Avtor), Majda Golob (Avtor), Bojan Papić (Avtor), Urška Dermota (Avtor), Irena Grmek-Košnik (Avtor), Darja Kušar (Avtor), Matjaž Ocepek (Avtor), Irena Zdovc (Avtor)

Povzetek

Abstract Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) represents a concern in both human and veterinary medicine. The aim of this study was to investigate potential LA-MRSA transmission between animals and humans in rural settings. To this aim, a study was designed to include 14 farms in Slovenia, which were selected on the basis of a farmer (initial patient) with confirmed LA-MRSA infection and regular animal contacts. On all farms, the initial patients, their household members, animals and barn environment were analysed for the presence of LA-MRSA. In addition, the epidemiologically linked hospital-related LA-MRSA isolates were included to investigate possible nosocomial transmissions. On five farms, LA-MRSA was discovered both in animals and in humans. In total, 49 LA-MRSA isolates of different origins underwent whole-genome sequencing, antimicrobial susceptibility testing and spa typing. All 49 isolates belonged to the sequence type 398 (ST398), spa types t011 and t034, and harboured staphylococcal chromosomal cassette mec Vc. High levels of concordance between resistance phenotypes and genotypes were observed. No transmission pairs between animals and initial patients were discovered. However, several isolates originating from farm animals and other household members formed clusters with pairwise distances of ≤14 single nucleotide polymorphisms (SNPs), indicating recent transmission events. In addition, three closely related isolates (0 SNP) form hospitalized patients were observed, indicating a possible nosocomial transmission. Two hospital-related isolates harboured the immune evasion cluster genes, which are associated with adaptation to the human host; however, these two isolates differed in >30 SNPs from the remaining isolates. Characteristics of LA-MRSA from Slovenia reflect those observed previously in other European studies. Immune evasion cluster-positive LA-MRSA ST398 suggests its re-adaptation to the human host and calls for a closer monitoring of such emerging LA-MRSA lineages, in addition to monitoring and preventing the introduction of LA-MRSA from farms to hospitals where transmission is highly plausible.

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Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL VF - Veterinarska fakulteta
UDK: 579
COBISS: 27039235 Povezava se bo odprla v novem oknu
ISSN: 1865-1674
Št. ogledov: 275
Št. prenosov: 36
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

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Sekundarne ključne besede: Communicable diseases, emerging;Microbiology;Veterinary;Methicillin-resistant Staphylococcus aureus;Staphylococcal infections;Epidemiology;Transmission;Livestock;Humans;Whole genome sequencing;Molecular typing;
Vrsta dela (COBISS): Članek v reviji
Konec prepovedi (OpenAIRE): 2022-03-20
Strani: str. 789-801
Letnik: ǂVol. ǂ68
Zvezek: ǂno. ǂ2
Čas izdaje: 2021
DOI: 10.1111/tbed.13745
ID: 13116218