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Št. zadetkov: 6
Persistence and Fab-specific interaction of acyl-protein thioesterase-1 with monoclonal antibodies during downstream processing
Ernest Šprager, Ernest Šprager, Aleksander Krajnc, Aleksander Krajnc, Mojca Lunder, Mojca Lunder, Jožica Vašl, Jožica Vašl, Tomaž Bratkovič, Tomaž Bratkovič
Izvirni znanstveni članek
Oznake: monoclonal antibodies;chromatography;host cell proteins;acyl-protein thioesterase-1;protein-protein interactions;biolayer interferometry;
Difficult-to-remove host cell proteins (HCPs) can compromise monoclonal antibody (mAb) product quality and stability. This study investigated the persistence of acyl-protein thioesterase-1, a polysorbate-degrading HCP, during downstream processing and its interaction with a set of mAbs. Acyl-protein ...
Leto: 2026 Vir: Fakulteta za farmacijo (UL FFA)
Produkcija in karakterizacija modificiranih oblik proteinskih plinskih veziklov v E. coli
Ernest Šprager, Mojca Benčina, Marko Novinec, Brigita Lenarčič, Gregor Gunčar
Magistrsko delo
Oznake: E. coli;proteinski plinski vezikli;proteini;GvpC;mutageneza skupka gvp;ultrazvok;magistrska dela;
Proteinski plinski vezikli so cilindrične ali vretenaste votle nanostrukture prokariontskih mikroorganizmov s premerom 45 – 200 nm in dolžino 100 – 200 nm, napolnjene s plini in obdane s proteinsko steno, debelo 2 nm. Glavni strukturni protein predstavlja GvpA (ali paralogni GvpB), pri določenih vrs ...
Leto: 2020 Vir: Fakulteta za kemijo in kemijsko tehnologijo (UL FKKT)
Efficient host cell protein clearance
Ernest Šprager, Veronika Reisinger, Jonas Sommer, Tinkara Lekić, Nina Pucelj, Tina Kljun, Mojca Lunder, Jožica Vašl, Tomaž Bratkovič
Izvirni znanstveni članek
Oznake: anion-exchange chromatography;host cell proteins;aggregates;polysorbate degradation;design of experiments;membrane adsorbers;
In recent years, biopharmaceutical purification processes have shifted towards more productive and cost-effective methods. Membrane technology has emerged as viable alternative to conventional resins, capable of delivering similar product quality while facilitating a simpler and more flexible downst ...
Leto: 2025 Vir: Fakulteta za farmacijo (UL FFA)
Protein gas vesicles of bacillus megaterium as enhancers of ultrasound-induced transcriptional regulation
Vid Jazbec, Nina Varda, Ernest Šprager, Maja Meško, Sara Vidmar, Rok Romih, Marjetka Podobnik, Andreja Kežar, Roman Jerala, Mojca Benčina
Izvirni znanstveni članek
Oznake: biokemija;beljakovine;proteini;plinski vezikli;bakterije;
Gas vesicles (GVs) are large cylindrical gas-filled protein assemblies found in diverse aquatic bacteria that enable their adaptation of buoyancy. GVs have already been used as ultrasound contrasting agents. Here, we investigate GVs derived from Bacillus megaterium, aiming to minimize the number of ...
Leto: 2024 Vir: Medicinska fakulteta (UL MF)
ǂA ǂmultifaceted approach to understanding protein-buffer interactions in biopharmaceuticals
Blaž Lebar, Maria Toplishek, Boštjan Japelj, Ernest Šprager, Rok Podlipec, Tilen Knaflič, Iztok Urbančič, Benjamin Knez, Mitja Zidar, Jure Cerar, Janez Mravljak, Aleš Žula, Denis Arčon, Janez Plavec, Stane Pajk
Izvirni znanstveni članek
Oznake: biokemija;jedrska magnetna resonanca;NMR;molekulska dinamika;
Leto: 2024 Vir: Kemijski inštitut (KI)
ǂA ǂdeep dive into spin-labeled polysorbate’s interaction with therapeutic antibody using 2D NMR, EPR and MD simulations
Blaž Lebar, Maria Toplishek, Boštjan Japelj, Ernest Šprager, Rok Podlipec, Tilen Knaflič, Iztok Urbančič, Benjamin Knez, Mitja Zidar, Jure Cerar, Janez Mravljak, Aleš Žula, Denis Arčon, Janez Plavec, Stane Pajk
Izvirni znanstveni članek
Oznake: monoklonsko protitelo;interakcija polisorbat;jedrska magnetna resonanca;elektronska paramagnetna resonanca;molekularna dinamika;monoclonal antibody;interaction polysorbate;nuclear magnetic resonance;electron paramagnetic resonance;molecular dynamics;
Polysorbates (PS) are widely used surfactants in biopharmaceutical formulations playing a crucial role in protecting proteins against mechanical stress and interface-induced damage. However, their susceptibility to degradation can compromise their function and lead to particle formation. Recent stud ...
Leto: 2025 Vir: Fakulteta za farmacijo (UL FFA)
Št. zadetkov: 6
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