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Št. zadetkov: 8
Genetic diversity of deformed wing virus from Apis mellifera carnica (Hymenoptera:Apidae) and varroa mite (Mesostigmata: Varroidae)
Urška Jamnikar, Metka Pislak, Ivan Toplak
Izvirni znanstveni članek
Oznake: bees;deformed wing virus - genetics;varroidae;phylogeny;
Deformed wing virus (DWV) is one of the most widespread viruses that infect honey bee colonies. The route of infection is directly through contaminated food, feces, and air, or indirectly through the varroa mite, which acts as a vector. Positive DWV samples were obtained from Carn ...
Leto: 2019 Vir: Veterinarska fakulteta (UL VF)
Determination of genetically identical strains of four honeybee viruses in bumblebee positive samples
Ivan Toplak, Laura Šimenc, Metka Pislak, Danilo Bevk
Izvirni znanstveni članek
Oznake: honeybees;bumblebees;viruses;sequencing;transmission;epidemiology;bees;
In recent years, there has been growing evidence that certain types of honeybee viruses could be transmitted between different pollinators. Within a voluntary monitoring programme, 180 honeybee samples (Apis mellifera carnica) were collected from affected apiaries between 2007 and 2018. Also from Au ...
Leto: 2020 Vir: Veterinarska fakulteta (UL VF)
ǂThe ǂpathogens spillover and incidence correlation in bumblebees and honeybees in Slovenia
Metka Pislak, Ivan Toplak, Urška Zajc, Danilo Bevk
Izvirni znanstveni članek
Oznake: bumblebees;honeybees;viruses;Nosema spp.;Crithidia bombi;Apicystis bombi;Lotmaria passim;pathogens transmission;animal diseases;bees;
Slovenia has a long tradition of beekeeping and a high density of honeybee colonies, but less is known about bumblebees and their pathogens. Therefore, a study was conducted to define the incidence and prevalence of pathogens in bumblebees and to determine whether there are links between infections ...
Leto: 2021 Vir: Nacionalni inštitut za biologijo (NIB)
First detection of honeybee pathogenic viruses in butterflies
Metka Pislak, Gordana Glavan, Rudi Verovnik, Laura Šimenc, Ivan Toplak
Izvirni znanstveni članek
Oznake: butterflies;honeybees;viruses;pathogens transmission;bees;virology;
Several pathogens are important causes of the observed pollinator decline, some of which could be transmitted between different pollinator species. To determine whether honeybee viruses can be transmitted to butterflies, a total of 120 butterflies were sampled at four locations in Slovenia. At each ...
Leto: 2022 Vir: Veterinarska fakulteta (UL VF)
Using whole-genome sequencing to assess the diversity of Paenibacillus larvae within an outbreak and a beekeeping operation
Bojan Papić, Majda Golob, Irena Zdovc, Jana Avberšek, Metka Pislak, Darja Kušar
Izvirni znanstveni članek
Oznake: Paenibacillus larvae;American foulbrood;AFB;whole-genome sequencing;WGS;whole-genome multilocus sequence typing;wgMLST;outbreak;beekeeping operation;molecular sequence data;genotype;
The spore-forming bacterium Paenibacillus larvae is the causative agent of American foulbrood (AFB), a devastating disease of honeybees (Apis mellifera). In the present study, we used whole-genome sequencing (WGS) to investigate an extensive outbreak of AFB in northwestern Slovenia in 2019. A total ...
Leto: 2021 Vir: Veterinarska fakulteta (UL VF)
Predlog načrta za hitro odzivanje ob najdbi azijskega sršena (Vespa velutina)
Maarten De Groot, Simon Zidar, Danilo Bevk, Mojca Pibernik, Metka Pislak, Jana Kus Veenvliet
Elaborat, predštudija, študija
Oznake: azijski sršen;invazivni organizmi;tujerodni organizmi;
A proposal of a rapid response plan for the Asian hornet (Vespa velutina) was prepared in the LIFE ARTEMIS Project. The Asian hornet is an invasive alien species which is on the priority list of the IAS EU legislation 1143/2014. In the proposal the EWRR system is described for the finding and the ra ...
Leto: 2020 Vir: Nacionalni inštitut za biologijo (NIB)
ǂThe ǂdiagnostic value of qPCR quantification of Paenibacillus larvae in hive debris and adult bees for predicting the onset of American foulbrood
Bojan Papić, Lucija Poljanšek, Metka Pislak, Barbara Hočevar, Monika Kozar, Rene Rus, Urška Zajc, Darja Kušar
Izvirni znanstveni članek
Oznake: Paenibacillus larvae;American foulbrood;quantitative PCR;logistic regression;
American foulbrood (AFB) is a serious infectious disease of honeybees (Apis mellifera) caused by Paenibacillus larvae. Increased P. larvae count in hive-related material is associated with an increased risk of AFB. Here, we quantified P. larvae cells in 106 adult bee and 97 hive debris samples using ...
Leto: 2024 Vir: Veterinarska fakulteta (UL VF)
Novel TaqMan PCR assay for the quantification of Paenibacillus larvae spores in bee-related samples
Darja Kušar, Bojan Papić, Urška Zajc, Irena Zdovc, Majda Golob, Lucija Poljanšek, Tanja Knific, Jana Avberšek, Matjaž Ocepek, Metka Pislak
Izvirni znanstveni članek
Oznake: American foulbrood (AFB);Paenibacillus larvae;real-time or quantitative PCR (qPCR);digital PCR (dPCR);plate counting;spore germination rate;honey;hive debris;Polymerase Chain Reaction;
Paenibacillus larvae is the causative agent of American foulbrood (AFB), a devastating disease of honeybees. P. larvae spore counts in bee-related samples correlate with the presence of AFB symptoms and may, therefore, be used to identify at-risk colonies. Here, we constructed a TaqMan-based real-ti ...
Leto: 2021 Vir: Veterinarska fakulteta (UL VF)
Št. zadetkov: 8
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